Screening of Marine Actinomycetes from Segara Anakan for Natural Pigment and Hydrolytic Activities

Publons ID18321210
Wos IDWOS:000372053500056
Doi10.1088/1757-899X/107/1/012056
TitleScreening of Marine Actinomycetes from Segara Anakan for Natural Pigment and Hydrolytic Activities
First Author
Last Author
AuthorsAsnani, A; Ryandini, D; Suwandri;
Publish Date2016
Journal Name10TH JOINT CONFERENCE ON CHEMISTRY
Citation1
AbstractMarine actinomycetes have become sources of great interest to natural product chemistry due to their new chemical entities and bioactive metabolites. Since April 2010, we have screened actinobacteria from five sites that represent different ecosystems of Segara Anakan lagoon. In this present study we focus on specific isolates, K-2C which covers 1) actinomycetes identification based on morphology observation and 16S rRNA gene; 2) fermentation and isolation of pigment; 3) structure determination of pigment; and 4) hydrolytic enzymes characterization; Methodologies relevant to the studies were implemented accordingly. The results indicated that K-2C was likely Streptomyces fradiae strain RSU15, and the best fermentation medium should contain starch and casein with 21 days of incubation. The isolate has extracellular as well as intracellular pigments. Isolated pigments gave purple color with lambda(max) of 529.00 nm. The pigment was structurally characterized. Interestingly, Streptomyces K-2C was able to produce potential hydrolytic enzymes such as amylase, cellulase, protease, lipase, urease, and nitrate reductase.
Publish TypeBook in series
Publish Year2016
Page Begin(not set)
Page End(not set)
Issn1757-8981
Eissn
Urlhttps://www.webofscience.com/wos/woscc/full-record/WOS:000372053500056
AuthorARI ASNANI, S.Si, M.Sc., Ph.D
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