| Publons ID | (not set) |
|---|
| Wos ID | WOS:001349647700002 |
|---|
| Doi | 10.3746/pnf.2024.29.3.345 |
|---|
| Title | Precooked Jack Bean [Canavalia ensiformis (L.) DC] Sprout: Generation of Dipeptidyl Peptidase-IV Inhibitory Peptides during Simulated Digestion |
|---|
| First Author | |
|---|
| Last Author | |
|---|
| Authors | |
|---|
| Publish Date | SEP 2024 |
|---|
| Journal Name | PREVENTIVE NUTRITION AND FOOD SCIENCE |
|---|
| Citation | |
|---|
| Abstract | Bioactive peptides generated from jack bean sprouts are reported to function as dipeptidyl peptidase IV (DPP- IV) inhibitors. However, no studies have investigated the effect of precooking followed by simulated digestion using pepsinpancreatin to increase DPP-IV inhibitory peptide generation in jack bean sprouts. Therefore, the present study aimed to explore the generation of DPP-IV inhibitory peptides from precooked jack bean [ Canavalia ensiformis (L.) DC] sprouts during simulated digestion with pepsin-pancreatin. The results showed that peptide fractions of the sample hydrolysate with molecular weight <1 kDa exhibited the strongest DPP-IV inhibitory activity (84.77%+/- 0.49%) after simulated digestion. This activity was slightly greater than that (74.12%+/- 0.85%) observed prior to simulated digestion. These findings demonstrate that the DPP-IV inhibitory activity of precooked jack bean sprouts can be retained following simulated digestion. Moreover, our investigation revealed the sequences of two novel peptides following simulated digestion with critical amino acids. The presence of alanine and glycine at the penultimate N-terminus of AAGPKP and LGDLLK confirmed the presence of DPP-IV inhibitors. Both peptide sequences are nontoxic and interact with the catalytic sites of enzymes through hydrogen bonds. |
|---|
| Publish Type | Journal |
|---|
| Publish Year | 2024 |
|---|
| Page Begin | 345 |
|---|
| Page End | 353 |
|---|
| Issn | 2287-1098 |
|---|
| Eissn | 2287-8602 |
|---|
| Url | https://www.webofscience.com/wos/woscc/full-record/WOS:001349647700002 |
|---|
| Author | Dr FRISKA CITRA AGUSTIA, S.TP, M.Sc. |
|---|
| File | 165482.pdf |
|---|