| Publons ID | 17899352 |
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| Wos ID | WOS:000399169500068 |
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| Doi | 10.1088/1757-899X/172/1/012068 |
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| Title | <i>Bacillus thuringiensis</i> HCB6 Amylase Immobilization by Chitosan Beads |
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| First Author | Zusfahair; Ningsih, D. R.; Kartika, D.; |
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| Last Author | Zuliana, A. L. |
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| Authors | Zusfahair; Ningsih, DR; Kartika, D; Fatoni, A; Zuliana, AL; |
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| Publish Date | 2017 |
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| Journal Name | MATERIAL CHEMISTRY DEVELOPMENT FOR FUTURE MEDICINE, INDUSTRY, ENVIRONMENTAL AND BIOMATERIAL APPLICATION |
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| Citation | 4 |
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| Abstract | The purpose of this study was to optimize the amylase immobilization using a chitosan bead and to characterize immobilized amylase of Bacillus thuringiensis Bacteria HCB6. This study was started of amylase production, continued by immobilization optimization including ratio of chitosan:enzymes, enzyme-matrix contact time, substrate concentration, pH effect, incubation temperature effect, reaction time, and stability of immobilized enzyme. Amylase activity assay was dinitro salicylic (DNS) method. The results showed the optimum chitosan:enzyme ratio was 2.5: 1 (v/v), immobilization contact time of 18 hours and immobilization efficiency of 87.93%. Furthermore, immobilized amylase of B. thuringiensis HCB6 showed optimum substrate concentration of 1.5%, optimum pH of 6, optimum incubation temperature of 37 degrees C, and the reaction time of 30 minutes. The Michaelis-Menten constant KM value for free and immobilized amylase were 5.30% and 1.33% respectively. Immobilized amylase can be used up to five times with the remaining activity of 43.3%. |
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| Publish Type | Book in series |
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| Publish Year | 2017 |
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| Page Begin | (not set) |
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| Page End | (not set) |
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| Issn | 1757-8981 |
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| Eissn | |
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| Url | https://www.webofscience.com/wos/woscc/full-record/WOS:000399169500068 |
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| Author | DWI KARTIKA, S.Si, M.Sc. |
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| File | 15762.pdf |
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