Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda 'Saint Valentine': Application of NAA and TDZ

Publons ID(not set)
Wos IDWOS:000844515100001
Doi10.13057/nusbiosci/n140205
TitleSomatic embryogenesis of the selected intergeneric hybrid between <i>Phalaenopsis</i> 2166 and <i>Vanda</i> 'Saint Valentine': Application of NAA and TDZ
First Author
Last Author
AuthorsDwiati, M; Susanto, AH; Budisantoso, I;
Publish DateNOV 2022
Journal NameNUSANTARA BIOSCIENCE
Citation
AbstractAn intergeneric hybridization between Phalaenopsis 2166 and Vanda 'Saint Valentine' has successfully produced a hybrid seedling with several characters of potentially developing into plant individuals with flowers of better performance. Therefore, identical clones of the selected hybrid should be developed into PLBs by means of in-vitro culture technique employing somatic embryogenesis supported by the application of plant growth regulators. This study aims to unveil the effect of NAA and TDZ in stimulating the formation of identical clones of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda 'Saint Valentine'. The experiment was arranged in a factorial Randomized Complete Block Design (RCBD) involving two factors, i.e., types of plant growth regulators and the levels of concentrations of each substance. It was found that the combination of NAA and TDZ had significant effect on the growth of the identical clones. The combination of NAA 0.5 mgL(-1) and TDZ 1.5 mgL(-1) resulted in the clones that potentially differentiate into PLBs. This finding indicates that NAA and TDZ should be applied appropriately to stimulate somatic embryogenesis in the intergeneric hybrid.
Publish TypeJournal
Publish Year2022
Page Begin160
Page End165
Issn2087-3948
Eissn2087-3956
Urlhttps://www.webofscience.com/wos/woscc/full-record/WOS:000844515100001
AuthorDr Dra MURNI DWIATI, MSi
File108954.pdf