| Title | In Vitro Antioxidant Activity of Zingiber officinale, Piper retrofractum, and Their Combinations |
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| Author Order | 4 of 7 |
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| Accreditation | |
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| Abstract | Many plants are used empirically as antioxidant. Plants that are frequently used in Indonesian communities are Zingiber officinale and Piper retrofractum. The aim of this research was to investigate the in vitro antioxidant activity of single ethanolic extract and the combinations ofZ. officinale and P. retrofratum using free radical scavenging DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Z. officinale and P. retrofratum were extracted by maceration using 95% ethanol for 3 x 24 hours.ÃÂàAntioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The concentration of the extract (ÃÂüg/mL) that was required to scavenge 50% of free radicals (IC50)was calculatedusing the percent scavenging activities of six different extract concentrations. The results showed that the single ethanolic extract of Z. Officinalle produced the highest antioxidant activity with IC50of 56 ÃÂüg/mL, while the antioxidantÃÂàactivity of the single ethanolic extract ofÃÂàP. retrofractum produced an IC50 of 3.445 ÃÂüg/mL. The IC50 of combination of Z. officinale and P. retrofratum ethanolic extracts at concentration ratios of 1: 2, 1 : 1, and 2 : 1 were 148 ÃÂüg/mL, 85 ÃÂüg/mL, and 73 ÃÂüg/mL.Keywords: Zingiber officinale, Piper retrfratum, Antioxidant, DPPH |
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| Publisher Name | Indonesian Society for Cancer Chemoprevention |
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| Publish Date | 2011-10-31 |
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| Publish Year | 2011 |
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| Doi | DOI: 10.14499/indonesianjcanchemoprev2iss3pp295-298 |
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| Citation | 1 |
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| Source | Indonesian Journal of Cancer Chemoprevention |
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| Source Issue | Vol 2, No 3 (2011) |
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| Source Page | 295-298 |
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| Url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/46/47 |
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| Author | Dr.nat.techn Apt HENDRI WASITO, S.Farm, M.Sc. |
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| File | 972723.pdf |
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