| Title | Metalloproteinase-9 gene variants and risk for hypertension among ethnic Javanese |
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| Author Order | 3 of 3 |
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| Accreditation | |
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| Abstract | BackgroundHypertension is associated with endothelial-dependent vasodilation disorders, due to reduced nitric oxide (NO) availability and excessive angiotensin II (ANG-II) activation. The objective of this study was to determine the association between matrix metallopeptidase 9 (MMP-9) gene polymorphism and hypertension in ethnic Javanese in the 40-80 year age group. MethodsThis was a case-control study on 50 PROLANIS patients of family doctors meeting the inclusion criteria and 50 controls without hypertension. Subjects were hypertensive patients with constant systolic arterial pressure of >140 mmHg and diastolic arterial pressure of >90 mmHg, confirmed in three successive measurements The observed parameters were degree of MMP-9 polymorphism, and NO and ANG-II levels. Matrix metallopeptidase 9 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using the SmaI restriction enzyme. MMP-9 polymorphisms were indicated by variation in band patterns. Degree of polymorphism in cases and controls were compared with NO and ANG-II levels in both groups. Data analysis was done using independent t-test.ResultsThe heterozygous (3 band) to normal (2 band) MMP-9 genotype ratio was 3:1 in hypertensives, but balanced in controls. In hypertensives, heterozygous GA and homozygous AA genotype frequencies were respectively 3.198 and 1.548 times higher than that of the GG genotype (p=0.008 and p=0.726). There was a statistically significant differences of NO and Ang-II levels between cases and controls (p=0.000 and p=0.000; respectively). ConclusionMatrix metallopeptidase 9 gene polymorphisms in hypertensive ethnic Javanese are associated with NO and angiotensin II levels. |
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| Publisher Name | Faculty of Medicine, Universitas Trisakti |
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| Publish Date | 2014-12-02 |
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| Publish Year | 2014 |
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| Doi | DOI: 10.18051/UnivMed.2014.v33.213-220 |
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| Citation | |
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| Source | Universa Medicina |
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| Source Issue | Vol. 33 No. 3 (2014) |
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| Source Page | 213-220 |
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| Url | https://univmed.org/ejurnal/index.php/medicina/article/view/36/30 |
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| Author | dr. dr ALFI MUNTAFIAH, M.Sc. |
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| File | 494052.pdf |
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