Garuda Documents : Phytochemical screening and purification of n-hexane fraction of Calophyllum soulattri leaves

TitlePhytochemical screening and purification of n-hexane fraction of Calophyllum soulattri leaves
Author Order5 of 6
Accreditation
AbstractBackground: Calophyllum soulattri Burm F. is widely utilized in traditional medicine. It is necessary to identify secondary metabolites from C. soulattri leaves to determine the pharmacologically active chemicals. Objective: This study aimed to screen the phytochemical content and purify the n-hexane fraction of C. soulattri leaves from Banyumas, Indonesia. Methods: The n-hexane fraction was macerated with methanol, followed by liquid-liquid fractionation with n-hexane. The n-hexane fraction was tested for flavonoids, triterpenoids/steroids, saponins, and phenols using the test tube method. In addition, the compounds were purified using column chromatography. The purified compound was identified by the Liebermann-Burchard reagent, which was compared with commercially available steroid drugs as reference. Results: Phytochemical analysis revealed that the n-hexane fraction of C. soulattri leaves contained secondary metabolites such as flavonoid, steroid, and phenol compounds. Analyses with the Liebermann-Burchard reagent indicated that the purified compound was potentially a steroid. Conclusion: The compound extracted from the n-hexane fraction of C. soulattri leaves was expected as a steroid.
Publisher NamePharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia
Publish Date2022-08-01
Publish Year2022
DoiDOI: 10.20884/1.api.2022.10.2.5858
Citation
SourceActa Pharmaciae Indonesia : Acta Pharm Indo
Source IssueVol 10 No 2 (2022): Acta Pharmaciae Indonesia : Acta Pharm Indo
Source Page5858
Urlhttp://jos.unsoed.ac.id/index.php/api/article/view/5858/3338
AuthorApt TRIYADI HENDRA WIJAYA, S.Farm, M.Si
File3181172.pdf