Garuda Documents : ANALISIS PROTEIN ISOLAT BAKTERI Escherichia coli BL 21 MENGGUNAKAN Sodium Dodecyl Sulphate – Polyacrylamide Gel Elektrophoresis (SDS-PAGE)

TitleANALISIS PROTEIN ISOLAT BAKTERI Escherichia coli BL 21 MENGGUNAKAN Sodium Dodecyl Sulphate – Polyacrylamide Gel Elektrophoresis (SDS-PAGE)
Author Order1 of 2
Accreditation
AbstractProtein is a structural and functional component found in living organisms. This study aims to determine the quantity of protein obtained and the type of protein that was successfully extracted from the culture of Escherichia coli BL 21 (DE3). The research method uses exploratory descriptive, measurement of protein concentration by spectrophotometer and analysis of protein molecular weight by SDS-PAGE electrophoresis. The results of the study it was found that the total protein isolated from E. coli BL21 (DE3) bacterial culture had a concentration of 7.67 µg / µl, the control concentration was higher than the sample which was 9.33 µg / µl. The conclutions of the study it was found that the concentration of total protein isolated from E. coli BL21 (DE3) bacterial culture had a of 7.67 µg/µl, but the molecular weight and type of protein in total protein were still unknown.
Publisher NameBiology Education Department, Faculty of Teacher Training and Education, University of Nahdlatul Wathan Mataram - Indonesia
Publish Date2019-09-30
Publish Year2019
Doi
Citation
SourcePENBIOS: JURNAL PENDIDIKAN BIOLOGI DAN SAINS
Source IssueVol 4 No 01 (2019): PENBIOS: Jurnal Pendidikan Biologi dan Sains, Vol. 4, No. 1, Mei 2019 pISSN 2541
Source Page1-7
Urlhttp://ejournal.unwmataram.ac.id/bios/article/view/160/107
AuthorEKA OKTAVIANI, S.Si, M.Biotech
File1376459.pdf