| Abstract | The ability ofÃÂ Peryonix excavatusÃÂ to live in extremely dirty area indicates thatÃÂ P. excavatusÃÂ secretes distinctive enzymes which might be useful for industry. Thus, this research were aimed to isolate amylase, lipase and protease fromÃÂ P. excavatus, and to characterize the enzymes to know the optimum temperature and pH. The isolation procedure consisted of extraction and ammonium sulphate fractionation. The results showed that crude extract and ammonium sulphate fractions ofÃÂ P.excavatusÃÂ had amylase, lipase, and protease enzymes activities. Among the three enzymes, amylase had the highest enzymatic activity whereas lipase was the least. The optimum temperature of amylase, lipase and protease were 60, 40, and 60ÃÂ oC, respectively. The optimum pH of amylase, lipase and protease were 7, 7, and 8, respectively. |
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