| Title | SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL |
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| Author Order | of |
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| Accreditation | |
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| Abstract | Microneme protein (MIC) is one of proteins that belongs toÃÂ excretory-secretory antigensÃÂ (ESAs) ofÃÂ Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of this research is to cloneÃÂ mic3ÃÂ (gene encoding for MIC-3) ofÃÂ T. gondiiÃÂ from local isolate using recombinant DNA technology by cloningÃÂ mic3ÃÂ in an expression vector.ÃÂ Deoxyribonucleic acidÃÂ (DNA) fromÃÂ T. gondiiÃÂ tachyzoites was amplified byÃÂ PuRe Taq RTG-PCR BeadsÃÂ usingÃÂ mic3ÃÂ specific primers. Amplified DNA was double digested usingÃÂ EcoRV andÃÂ HindIII restriction endonucleases and then purified usingÃÂ EZ-10 spin coloumn purification kit. TheÃÂ mic3 DNA was ligated into pET-32a(+) expression vector and transformated intoÃÂ Escherichia coliÃÂ BL21. The results showed that recombinantÃÂ mic3gene 4.2 kDa has been successfully performed by cloning gene encoding for MIC-3 protein ofÃÂ T. gondii local isolate into pET-32a(+) and transformed toÃÂ E. coliÃÂ BL21. |
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| Publisher Name | Universitas Jenderal Soedirman |
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| Publish Date | 2011-05-01 |
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| Publish Year | 2011 |
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| Doi | DOI: 10.20884/1.jm.2011.6.1.85 |
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| Citation | |
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| Source | Molekul |
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| Source Issue | Vol 6, No 1 (2011) |
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| Source Page | 10-18 |
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| Url | https://ojs.jmolekul.com/ojs/index.php/jm/article/view/85 |
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| Author | DIANA INDRASANTI, M.Biotech |
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| File | 1251702.pdf |
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