| Publons ID | 53445286 |
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| Wos ID | WOS:000318418000009 |
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| Doi | 10.1088/1742-6596/423/1/012009 |
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| Title | Analysis of Fluconazole in Human Urine Sample by High Performance Liquid Chromatography Method |
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| First Author | |
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| Last Author | |
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| Authors | Hermawan, D; Ali, NAM; Ibrahim, WAW; Sanagi, MM; |
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| Publish Date | 2013 |
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| Journal Name | 2013 INTERNATIONAL CONFERENCE ON SCIENCE & ENGINEERING IN MATHEMATICS, CHEMISTRY AND PHYSICS (SCIETECH 2013) |
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| Citation | 4 |
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| Abstract | A method for determination of fluconazole, antifungal drug in human urine by using reversed-phased high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was developed. Optimization HPLC conditions were carried out by changing the flow rate and composition of mobile phase. The optimum separation conditions at a flow rate 0.85 mL/min with a composition of mobile phase containing methanol: water (70: 30, v/v) with UV detection at a wavelength 254 nm was able to analyze fluconazole within 3 min. The excellent linearity was obtained in the range of concentration 1 to 10 mu g/mL with r(2) = 0.998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.39 mu g/mL and 1.28 mu g/mL, respectively. Solid phase extraction (SPE) method using octadecylsilane (C18) as a sorbent was used to clean-up and pre-concentrated of the urine sample prior to HPLC analysis. The average recoveries of fluconazole in spiked urine sample was 72.4% with RSD of 3.21% (n=3). |
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| Publish Type | Books in series |
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| Publish Year | 2013 |
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| Page Begin | (not set) |
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| Page End | (not set) |
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| Issn | 1742-6588 |
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| Url | https://www.webofscience.com/wos/woscc/full-record/WOS:000318418000009 |
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| Author | DADAN HERMAWAN, S.Si, M.Si, Ph.D |
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| File | 55701.pdf |
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